Abstract This work aimed to characterize the allspice leaves essential oil (EO) and evaluate its antimicrobial activity against specific food-borne pathogenic microorganisms as well as its in vitro antioxidant activity. The antioxidant activity of different concentrations (0, 500 and 1000 mg/kg) of allspice EO was also evaluated in mechanically deboned poultry meat (MDPM) during storage of up to 10 days at 2 °C. Allspice EO presented as major compounds eugenol (55.52%), myrcene (22.53%) and chavicol (5.12%), and was effective against Gram-negative (P. aeruginosa) and Gram-positive (Staphylococcus aureus) bacteria, having greater (p < 0.05) antimicrobial activity than its main component eugenol. In the in vitro antioxidant assays, the allspice EO had higher radical scavenging activity (90% versus 65%) than eugenol, presenting lower IC50 values (2.71 versus 9.49 µg/mL), but the antioxidant activity by the 2-thiobarbituric acid reactive substances (TBARS) did not differ (p > 0.05) from the synthetic antioxidant butyl-hydroxytoluene (BHT). Incorporating allspice EO in MDPM suppressed lipid oxidation during 8 days of cold storage, regardless of the amount used, exhibiting lower (p < 0.05) TBARS values during all storage periods than MDPM without EO. Allspice leaves EO had the high antioxidant potential to be used in MDPM and could also contribute an antimicrobial effect to the product in which the MDPM is used.

Resumo O objetivo deste trabalho foi caracterizar o óleo essencial (OE) de folhas de pimenta-da-jamaica e avaliar sua atividade antimicrobiana contra microrganismos patogênicos específicos de alimentos e sua atividade antioxidante in vitro. A ação antioxidante de diferentes concentrações (0, 500 e 1.000 mg/kg) de OE de pimenta-da-jamaica também foi avaliada em carne de frango desossada mecanicamente (MDPM) durante o armazenamento até 10 dias a 2 °C. O OE de pimenta-da-jamaica apresentou como compostos majoritários eugenol (55,52%), mirceno (22,53%) e chavicol (5,12%), e foi eficaz contra bactérias Gram-negativas (P. aeruginosa) e Gram-positivas (Staphylococcus aureus), tendo maior (p < 0,05) ação antimicrobiana do que seu principal componente, o eugenol. Nos ensaios antioxidantes in vitro, o OE de pimenta-da-jamaica apresentou maior atividade sequestrante de radicais (90% versus 65%) do que o eugenol, apresentando valores menores de IC50 (2,71 versus 9,49 µg/mL), mas a atividade antioxidante pelas substâncias reativas ao ácido 2-tiobarbitúrico (TBARS) não diferiu (p > 0,05) do antioxidante sintético butil-hidroxitolueno (BHT). A incorporação de OE de pimenta-da-jamaica no MDPM suprimiu a oxidação lipídica durante oito dias de armazenamento refrigerado, independentemente da quantidade utilizada, exibindo valores de TBARS menores (p < 0,05) durante todo o período de armazenamento do que o MDPM sem OE. O OE de folhas de pimenta-da-jamaica apresentou alto potencial antioxidante para ser utilizado em MDPM e também pode contribuir com um efeito antimicrobiano para o produto em que o MDPM é utilizado.

ABSTRACT The production of cachaça in Brazil is a historical and cultural practice that has been gaining ground in the commercial sector with the marketing of its product with new labels, derivatives and different types of packaging for the beverage. Polyethylene Terephthalate (PET) packaging has been used by some producers because of its lower cost and greater durability for transport. The influence of storage on 15 samples of commercial cachaça packed in polymeric packaging was investigated. The physical-chemical quality and the presence of Polycyclic Aromatic Hydrocarbons (PAH’s) in the beverages were evaluated. Of these samples, 60% contained concentrations of components that were outside the limits of the Quality Standards established by Ministry of Agriculture, Livestock and Supply (MAPA), and most of the samples contained low alcohol concentrations. The samples showed concentrations of ethyl carbamate below the limit established by MAPA (210 µg L-1). All samples studied contained concentrations of PAHs higher than those found in the literature for cachaça stored in other types of packages. Contamination by PAHs can be associated with the thermal processes involved in the production of packages. Sample R12 contained the highest total concentration of the PAHs (20.90 µg L-1), followed by samples R7 (17.23 µg L-1) and R8 (17.61 µg L-1). The mean concentration was 10.91 µg L-1. Fluorene was the PAH found in the largest quantity with a concentration of 18.61 µg L-1 in the R12 sample. Therefore, cachaças stored in PET packaging are unsuitable for consumption and commercialization, as they present high concentrations of contaminants.

RESUMO A produção de cachaça no Brasil é uma prática histórica e cultural que vem ganhando espaço no setor comercial com a comercialização de seu produto com novos rótulos, derivados e diferentes tipos de embalagens para a bebida. As embalagens de Polietileno Tereftalato (PET) têm sido utilizadas por alguns produtores devido ao seu menor custo e maior durabilidade para o transporte. A influência do armazenamento em 15 amostras de cachaça comercial acondicionada em embalagens poliméricas foi investigada. A qualidade físico-química e a presença de Hidrocarbonetos Policíclicos Aromáticos (HPA’s) nas bebidas foram avaliadas. Destas amostras, 60% continham concentrações de componentes que estavam fora dos limites dos Padrões de Qualidade estabelecidos pelo Ministério da Agricultura, Pecuária e Abastecimento (MAPA), e a maioria das amostras continha baixas concentrações de álcool. As amostras apresentaram concentrações de carbamato de etila abaixo do limite estabelecido pelo MAPA (210 µg L-1). Todas as amostras estudadas continham concentrações de HPA’s superiores às encontradas na literatura para cachaça armazenada em outros tipos de embalagens. A contaminação por HPA’s pode estar associada aos processos térmicos envolvidos na produção das embalagens. A amostra R12 continha a maior concentração total de HPA’s (20,90 µg L-1), seguida pelas amostras R7 (17,23 µg L-1) and R8 (17,61 µg L-1). A concentração média foi de 10,91 µg L-1. O fluoreno foi o HPA encontrado em maior quantidade com concentração de 18,61 µg L-1 na amostra R12. Portanto, as cachaças armazenadas em embalagens PET são impróprias para consumo e comercialização, pois apresentam altas concentrações de contaminantes.

RESUMO Ultimamente, no mercado de alimentos vem crescendo a demanda por produtos provenientes da agricultura orgânica. O Brasil lidera a produção de cachaça e produz cerca de 1,6 bilhões de litros/ano. Para a melhoria do produto, tem-se buscado novas tecnologias em toda a cadeia produtiva e utilizado matéria-prima orgânica na produção de cana-de-açúcar para a obtenção da bebida. O presente trabalho teve por objetivos traçar o perfil físico-químico e cromatográfico de onze amostras de cachaça orgânica provenientes de alguns estados brasileiros. Foram identificados e quantificados os componentes secundários e contaminantes por meio das análises físico-químicas, cromatografia líquida de alta eficiência (CLAE) e análises cromatográficas em fase gasosa (CG). Pelos resultados encontrados observou-se um percentual significativo das cachaças orgânicas pesquisadas, onde estas apresentaram parâmetros fora dos limites exigidos pelo Ministério da Agricultura, Pecuária e Abastecimento (MAPA), sendo encontrado valores inadequados de 18,20% para ésteres, 9,10% para cobre e extrato seco. Esta contaminação é acarretada pela má condução durante o processo pelos produtores, onde não estão observando as boas práticas de fabricação determinadas e/ou normatizadas pela legislação brasileira.

ABSTRACT There has been a growing demand for products from organic agriculture for the food market. Brazil leads the production of sugarcane spirits and produces about 1.6 billion liters/year. New technologies have been sought throughout the supply chain to improve production, and organic raw material has been used in the production of sugar cane for the production of beverages. This study aimed to define the physicochemical and chromatographic profiles of eleven organic sugarcane spirits samples from various Brazilian states. The secondary components and contaminants were identified and quantified through physicochemical analyses, HPLC and gas chromatography (GC). A significant percentage of the organic sugarcane spirits samples contained concentrations of components that were above the limits required by the Ministry of Agriculture, Livestock and Provisioning (MAPA), specifically the esters (18.20%), copper and dry extract (9.10%). This contamination is caused by bad conditions employed during the production process, which are not in compliance with the good manufacturing practices determined and legislated by Brazilian law.

RESUMO Um dos grandes problemas que a agricultura enfrenta é a perda de produção causada pelo ataque de pragas agrícolas. Assim, buscam-se maneiras alternativas de contornar esse problema. Dentre esses, encontram-se substâncias, denominadas de acilaçúcars, que são produzidas naturalmente por espécies selvagens do tomate S. pennellii e que apresentam efeito sobre artrópodes-praga. Os objetivos desse trabalho foram sintetizar dois compostos de acilaçúcares e avaliar o efeito biológico destas sobre os artrópodes-praga Bemissia tabaci e Tetranycus urticae. A síntese foi feita via as reações de glicose e sacarose com anidrido acético, utilizando acetato de sódio como catalisador. Os produtos dessas reações foram o octa-acetato de sacarose e o penta-acetato de glicose, cujas estruturas foram confirmadas por técnicas espectroscópicas. No teste de resistência do ácaro, foi possível obter um ajuste linear entre a concentração das substâncias sintetizadas e o deslocamento do ácaro. Ambas as substâncias obtidas apresentaram um atraso na eclosão dos ovos do artrópode, ocasionando uma mortalidade de aproximadamente 95% em ácaros de 1º ínstar que foram confirmadas em adultos. Nos testes biológicos com Bemisia tabaci, verificou-se uma baixa taxa de eclosão e emergência, sendo que o efeito sobre as ninfas foi a má formação para adultos emergidos.

ABSTRACT One of the main problems facing agriculture is the loss of production as a result of the attack of agricultural pests. Alternative ways to work around this problem are being sought. There are substances called acylsugars that are naturally produced by the wild tomato species S. pennellii and affect arthropod pests. The objectives of this work were to synthesize two acylsugars and assess the biological effect of these on the arthropod pests Bemissia tabaci and Tetranycus urticae. The syntheses were performed via the reactions of glucose and sucrose (saccharose) with acetic anhydride using sodium acetate as the catalyst. The products of these reactions were sucrose octa-acetate and glucose penta-acetate, the structures of which were confirmed by spectroscopic techniques. In a resistance test against the mite, a linear correlation between the concentration of the synthesized substances, and the dislocation of the mite was obtained. A delay in the hatching of the arthropod eggs was observed, causing a mortality rate of approximately 95% in the 1st instar larvae of mites that was confirmed in adults. In the biological tests with Bemisia tabaci, there was a low rate of hatching and emergence, and the effect on the nymphs was the deformation of the emergent adults.

BACKGROUND Recent studies showed that essential oils from different pepper species (Piper spp.) have promising leishmanicidal and trypanocidal activities. OBJECTIVES In search for natural compounds against Trypanosoma cruzi, different forms of the parasite were incubated for 24 h at 28ºC or 4ºC with Piper aduncum essential oil (PaEO) or its main constituents linalool and nerolidol. METHODS PaEO chemical composition was obtained by GC-MS. Drug activity assays were based on cell counting, MTT data or infection index values. The effect of PaEO on the T. cruzi cell cycle and mitochondrial membrane potential was evaluated by flow cytometry. FINDINGS PaEO was effective against cell-derived (IC50/24 h: 2.8 μg/mL) and metacyclic (IC50/24 h: 12.1 μg/mL) trypomastigotes, as well as intracellular amastigotes (IC50/24 h: 9 μg/mL). At 4ºC - the temperature of red blood cells (RBCs) storage in blood banks - cell-derived trypomastigotes were more sensitive to PaEO (IC50/24 h = 3.8 μg/mL) than to gentian violet (IC50/24 h = 24.7 mg/mL). Cytotoxicity assays using Vero cells (37ºC) and RBCs (4ºC) showed that PaEO has increased selectivity for cell-derived trypomastigotes. Flow cytometry analysis showed that PaEO does not affect the cell cycle of T. cruzi epimastigotes, but decreases their mitochondrial membrane potential. GC-MS data identified nerolidol and linalool as major components of PaEO, and linalool had trypanocidal effect (IC50/24 h: 306 ng/mL) at 4ºC. MAIN CONCLUSION The trypanocidal effect of PaEO is likely due to the presence of linalool, which may represent an interesting candidate for use in the treatment of potentially contaminated RBCs bags at low temperature.

RESUMO Os óleos essenciais apresentam possibilidade de serem empregados nas indústrias de alimentos, bebidas, produtos de higiene pessoal e cosméticos para evitar ou reduzir a deterioração lipídica e a contaminação por micro-organismos. Este trabalho teve como objetivos avaliar as propriedades funcionais antimicrobianas e antioxidantes de óleos essenciais de folhas frescas de Coniza bonariensis, Parthenium hysterophorus, Tithonia diversifolia, Ambrosia polystachya, Hedychium coronarium e Baccharis dracunculifolia, extraídos por hidrodestilação. O potencial antioxidante foi avaliado pelas metodologias do consumo do radical DPPH e da inibição da oxidação do sistema β-caroteno/ácido linoleico. A sensibilidade das bactérias Salmonella Cholerasuis, Listeria monocytogenes, Staphylococcus aureus e Escherichia coli frente aos óleos essenciais foi determinada pela utilização do método de difusão em cavidade ágar. Os óleos essenciais destacaram-se pelo elevado conteúdo de terpenoides. Todos os óleos essenciais avaliados pela metodologia do sequestro do radical DPPH não apresentaram CI50 significativos. Pela metodologia do β-caroteno/ácido linoleico, os óleos essenciais de T. diversifolia e H. coronarium não apresentaram atividades significativas e os de C. bonariensis, P. hysterophorus, A. polystachya, e B. dracunculifolia apresentaram CI50 superiores a maior concentração avaliada. Os óleos essenciais das espécies C. bonariensis, T. diversifolia, H. coronarium e de B. dracunculifolia apresentaram atividade antibacteriana para bactérias Gramnegativas e Gram-positivas, com exceção do óleo volátil de P. hysterophorus, que não impediu o crescimento de nenhuma das cepas bacterianas testadas. O óleo essencial de A. polystachya apresentou potencial antibacteriano apenas nas cepas de S. aureus.

ABSTRACT Essential oils can be employed in the food, drinks, toiletry and cosmetics industries to prevent or reduce lipid deterioration and contamination by microorganisms. The aim of this work was to evaluate the functional antimicrobial and antioxidant properties of essential oils of fresh leaves from Coniza bonariensis, Parthenium hysterophorus, Tithonia diversifolia, Ambrosia polystachya, Hedychium coronarium and Baccharis dracunculifolia, extracted by hydrodistillation. The methodologies used for assessing antioxidant potential were consumption of the DPPH radical and oxidation inhibition of the β-carotene/linoleic acid system. Sensitivity of the bacteria Salmonella cholerasuis, Listeria monocytogenes, Staphylococcus aureus and Escherichia coli to the essential oils was determined using the agar-well diffusion method. The essential oils were noteworthy for their high terpenoid content. None of the essential oils assessed by DPPH radical sequestration presented significant IC50. Using β-carotene/linoleic acid methodology, the essential oils of T. diversifolia and H. coronarium showed no significant activity, and C. bonariensis, P. hysterophorus, A. polystachya and B. dracunculifolia showed IC50 greater than the highest concentration evaluated. The essential oils of C. bonariensis, T. diversifolia, H. coronarium and B. dracunculifolia displayed antibacterial activity for gram-negative and grampositive bacteria, with the exception of the volatile oil of P. hysterophorus, which did not inhibit growth in any of the bacterial strains tested. The essential oil of A. polystachya displayed antibacterial activity only for strains of S. aureus.

ABSTRACT Essential oils are pharmacologically active and unexplored compounds. The inhibitory properties of essential oils from Baccharis dracunculifolia, Conyza bonariensis, Tithonia diversifolia and Ambrosia polystachya were evaluated in the coagulation and fibrinogenolysis induced by snake venoms. The essential oil from Conyza bonariensis extended the clotting time of Lachesis muta from 52.2 to 115.2 seconds and that of Bothrops moojeni from 108.3 to 2340.0 seconds, when pre-incubated with the venoms. The longest clotting times for Bothrops atrox venom were observed after incubation with the essential oils from Conyza bonariensis and Tithonia diversifolia: the times increased from 100.8 to 264.0 and 227.7 seconds, respectively. The prior incubation of the essential oils with plasma and subsequent addition of Lachesis muta venom resulted in a pro-clotting effect. The oils from Ambrosia polystachya and Baccharis dracunculifolia caused 100% of inhibition on the fibrinogenolysis induced by Bothrops moojeni and Lachesis muta venoms (the oils were previously incubated with the venom). The results indicate that the essential oils show promise as adjuvants for the treatment of snakebites.

RESUMOOs óleos essenciais apresentam potencial para serem empregados como bioherbicidas, possuindo vantagens em função da sua biodegradabilidade, elevada diversidade estrutural e reduzida resistência natural às plantas daninhas. Os óleos essenciais das folhas e dos rizomas de Hedychium coronarium, planta invasora exótica adaptada a diferentes regiões do Brasil, foram extraídos por hidrodestilação e caracterizados quimicamente por Cromatografia Gás-Líquido e Cromatografia Gás-Líquido/Espectrometria de Massas. As atividades alelopáticas foram determinadas pelas metodologias que avaliam os efeitos voláteis e o contato direto dos mesmos sobre a germinação de sementes e vigor de plântulas de alface. Os constituintes majoritários do óleo essencial das folhas foram o β-pineno (46,9%), o α-pineno (19,2%) e o β-cariofileno (13,2%) e o dos rizomas, o β-pineno (41,5%), o 1,8-cineol (23,6%) e o α-pineno (13,1%). Analisando os efeitos voláteis dos óleos essenciais, observou-se que o aumento das concentrações dos mesmos não alterou a primeira contagem de plântulas germinadas e a germinação total. Em relação ao IVG, à massa seca e ao comprimento das partes radiculares e aéreas das plântulas, o óleo essencial dos rizomas foi mais efetivo que o óleo essencial das folhas nas reduções da resposta das variáveis. Ao avaliar o efeito do contato direto dos óleos essenciais observou-se que ambos reduziram a resposta de todas as variáveis avaliadas e que, adicionalmente, o óleo dos rizomas provocou reduções superiores à do óleo das folhas em todas as variáveis. Estes resultados podem ser atribuídos ao maior conteúdo monoterpênico presente no óleo essencial dos rizomas, destacando-se a presença do 1,8-cineol.

ABSTRACTEssential oils have the potential to be used as bioherbicides, and possess the advantage of their biodegradability, high structural diversity and reduced natural resistance to weeds. The essential oils of the leaves and rhizomes of Hedychium coronarium, an exotic invasive plant adapted to different regions of Brazil, were extracted by hydrodistillation and characterised chemically by Gas-Liquid Chromatography and Gas-Liquid Chromatography/Mass Spectrometry. Allelopathic activity was determined using methodologies that evaluate the effects of volatility and direct contact on seed germination and seedling vigour in the lettuce. The major constituents of the essential oil from the leaves were β-pinene (46.9%), α-pinene (19.2%) and β-caryophyllene (13.2%) and from the rhizomes, β-pinene (41.5%), 1.8-cineole (23.6%) and α-pinene (13.1%). When analysing the volatile effects of the essential oils, it was seen that their concentration did not affect seedling first germination count or total germination. The essential oil from the rhizomes was more effective than the essential oil from the leaves in reducing seedling response for SGI, dry weight, and length of the roots and shoots. When evaluating the effect of direct contact with the essential oils, it was seen that both oils reduced the response of all the variables under evaluation, and that in addition, the oil from the rhizomes caused greater reductions than that from the leaves, again for all variables. These results can be attributed to the higher levels of monoterpenes present in the essential oil from the rhizomes, mainly the presence of 1.8-cineole.

This study had analyzed the antibacterial, antifungal and trypanocidal activity of the essential oils from Cinnamodendron dinisii Schwacke (Canellaceae) and Siparuna guianensis Aublet (Siparunaceae). The essential oils were obtained from fresh leaves by hydrodistillation, using a modified Clevenger apparatus. Chemical analysis by gas-liquid chromatography coupled to mass spectrometry (GC-MS) showed that these essential oils are rich in monoterpene and sesquiterpene hydrocarbons. Activity against the pathogenic bacteria Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella choleraesuis and Staphylococcus aureus was evaluated with the agar cavity diffusion method, while activity on the filamentous fungi Aspergillus flavus, Aspergillus niger, Aspergillus carbonariusand Penicillium commune was evaluated by the disk diffusion technique. Trypanocidal activity was tested against Trypanosoma cruzi epimastigotes, using the Tetrazolium salt (MTT) colorimetric assay. Both essential oils exhibited low inhibitory effect towards bacteria, showing high MIC values (125–500 μg mL−1), with Gram positive bacteria being more susceptible. Better inhibitory effect was obtained for the evaluated fungi, with lower MIC values (7.81–250 μg mL−1), being A. flavus the most susceptible species. Both essential oils presented low trypanocidal activity, with IC50/24 h values of 209.30 μg mL−1 for S. guianensis and 282.93 μg mL−1 for C. dinisii. Thus, the high values observed for the MIC of evaluated bacteria and for IC50/24 h of T. cruzi, suggest that the essential oils have a low inhibitory activity against these microorganisms. In addition, the low MIC values observed for the tested fungi species indicate good inhibitory activity on these microorganisms’s growth.

O aumento da produção e consumo de cachaça de alambique tem estimulado a pesquisa sobre a qualidade da bebida. Sabe-se que o cobre, quando encontrado em teor elevado na bebida e ingerido em grandes quantidades, pode causar danos à saúde humana. Assim, há uma grande preocupação por parte de pesquisadores e produtores quanto à utilização de substâncias adsorventes na remoção de seu excesso. Conduziu-se este trabalho, com os objetivos de estudar a cinética e a isoterma de adsorção de cobre em caulinitas e avaliar a qualidade físico-química da cachaça antes e após a adsorção. As caulinitas K01, K03 e K04 apresentaram capacidade máxima de adsorção do metal, 97, 95 e 90%, respectivamente. Observou-se que a cinética de adsorção segue o modelo de pseudoprimeira ordem e que os dados de isotermas se ajustaram ao modelo de Freundlich. Nos resultados das análises físico-químicas, todos os parâmetros da amostra controle estavam dentro dos limites exigidos pela legislação, exceto para os aldeídos e cobre. Após a adsorção com as argilas estudadas, a cachaça apresentou uma redução dos mesmos e para alguns parâmetros não houveram diferenças significativas. Pelos resultados da análise multivariada, com a primeira e a segunda componente principal, foi possível descrever 87% dos dados e pode-se constatar que todas as amostras diferiram-se do controle quanto aos parâmetros analisados. Assim, as argilas analisadas podem futuramente, após mais estudos, serem utilizadas na remoção do cobre da cachaça.

Increased production and cachaça consumption has stimulated research regarding its quality. It is known that copper can be harmful to human health when it is in concentrated form in the beverage and is ingested in large amounts. Thus, there is a great concern among researchers and producers about the use of adsorbent substances for the removal of excess copper. The kinetic parameters and the adsorption isotherms for copper on kaolinite were studied to evaluate the physical-chemical quality of the cachaça before and after adsorption. K01, K03 and K04 kaolinites presented a maximum adsorption capacity of 97, 95 and 90%, respectively. The kinetic rates followed the pseudo-first-order model, and the adsorption isotherm was best adjusted to the Freundlich model. All the parameters of the control samples were within the limits required by law, except for the aldehydes and copper. After adsorption with clay, the values for some of the parameters were lower and those of others were not significantly different. It was possible to describe 87% of the data with the first and second principal components of the multivariate analysis, and all the samples were different from the control for the parameters analyzed. Thus, these clays can be used for the removal of copper from cachaça, but further studies are necessary.

Tendo em vista as diversas atividades biológicas apresentadas pelos óleos essenciais e o potencial antibacteriano do óleo essencial de Lippia sidoides Cham., objetivou-se, com este trabalho, caracterizar o óleo essencial desta espécie coletada na região sul do estado de Minas Gerais-Brasil, bem como identificar as suas estruturas secretoras e avaliar sua atividade antimicrobiana sobre as fitobactérias Clavibacter michiganensis subsp. Michiganensis, Xanthomonas vesicatoria e Pseudomonas syringae pv. tomato. As plantas foram coletadas no município de Itumirim e os óleos essenciais de suas folhas, extraídos por hidrodestilação, utilizando-se um aparelho de Clevenger modificado. O óleo essencial apresentou como constituintes majoritários o carvacrol (26,44%) e o 1,8-cineol (22,63%). Nas superfícies foliares de L. sidoides foram encontrados tricomas glandulares e não glandulares. Entre os tricomas glandulares, encontram-se tricomas capitados do tipo I e tricomas peltados. Foram reveladas a presença de substâncias lipídicas e terpênicas no interior das células das cabeças dos tricomas peltados e capitados. apresentando as seguintes CMI: 7,81 µg mL-1 sobre C. michiganensis, 3,9 µg mL-1 sobre P. syringae e 15,62 µg mL-1 sobre X. vesicatoria. O carvacrol, seu constituinte majoritário, apresentou valores de CMI igual a 3,9 µg mL-1 sobre todas as três bactérias estudadas. Ambos demonstraram a capacidade de inibir o crescimento de bactérias Gram-positivas e Gram-negativas.

Given the diverse biological activities presented by essential oils, and the antibacterial potential of the essential oil of Lippia sidoides Cham, the objective of this work was to characterise the essential oil of this species, collected in the southern part of the state of Minas Gerais, Brazil, as well as to identify its secretory structures and evaluate its antimicrobial activity on the phytobacteria, Clavibacter michiganensis subsp. Michiganensis, Xanthomonas vesicatoria and Pseudomonas syringae pv. tomato. Plants were collected in the town of Itumirim, and the essential oils of the leaves extracted by hydrodistillation using a modified Clevenger apparatus. The essential oil presented carvacrol (26.44%) and 1.8-cineole (22.63%) as major constituents. On the leaf surface of L. sidoides both glandular and non-glandular trichomes were found. Among the glandular trichomes are peltate trichomes and type I capitate trichomes. The presence of lipidic and terpenic substances within the head cells of both the peltate and capitate trichomes was found. The essential oil of L. sidoides was able to inhibit the growth of all the microorganisms studied, showing the following MIC: 7.81 µg mL-1 for C. michiganensis, 3.9 µg mL-1 for P. syringae and 15.62 µg mL-1 for X. vesicatoria. Carvacrol, the major constituent, showed MIC values equal to 3.9 µg mL-1 for all three bacteria studied. Both exhibited the ability to inhibit the growth of gram-positive and gram-negative bacteria.

Este trabalho teve como objetivo identificar e quantificar os constituintes dos óleos essenciais de Illicum verum, Ageratum conyzoides,Piper hispidinervume Ocotea odorifera, bem como avaliar a toxicidade para o pulgão-verde Schizaphis graminum. A qualificação dos constituintes foi realizada por meio de um cromatógrafo gasoso + espectrômetro de massas, e a quantificação, por um cromatógrafo gasoso + detector de ionização de chama, ambos com uma coluna DB5. O método de hidrodestilação promoveu um rendimento (p/p) de 3,81% para I. verum, 0,46% para A. conyzoides, 2,85% para P. hispidinervume 0,68% para O. odorífera. Já os componentes majoritários foram: precoceno (87,0%) e (E)-cariofileno (7,1%) para A. conyzoides; (E)-anetol (90,4%), limoneno (2,6%) e metil-chavicol (1,3%) para I. verum; metil-eugenol (81,2%) e safrol (10,6%) para Ocotea odorífera; e safrol (82,5%) e α-terpinoleno (13,4%) para P. hispidinervum. Pelos testes de toxicidade aguda (24 horas) com folhas de sorgo ou papel-filtro contaminados, verificou-se que o óleo de A. conyzoides foi o mais tóxico para o pulgão, com CL50de 7,13 e 7,08 µL óleo/cm2 respectivamente, seguido por O. odorifera com CL50 de 11,80 e 103,00 µL óleo/cm2 respectivamente; I. verum de 51,80 µL óleo/cm2 em ambos os substratos; e o menos tóxico foi o óleo essencial de P. hispidinervum, com CL50 de 62,50 e 143,00 µL óleo/cm2, respectivamente. Dessa maneira, sugere-se que o uso dos óleos essenciais pode representar uma nova ferramenta em programas de manejo integrado de pragas.

The aim of this study was to assess the chemical composition of essential oils of Illicum verum, Ageratum conyzoides,Piper hispidinervumand Ocotea odorífera, as well as their toxicity to the green-aphid Schizaphis graminum. Compound identification was carried out with gas chromatography + mass spectrometry, and quantification with gas chromatography + flame ionization detector, both with DB5 column. The hydrodistillation process promoted oil yield of 3.81% for I. verum; 0.46% for A. conyzoides; 2.85 % for P. hispidinervum;and 0.68 % for O. odorífera. The major components from the oils of A. conyzoides was precocene (87.0%) and (E)-caryophyllene (7.1%); (E)-anethol (90.4%), limonene (2.6%) and methyl-chavicol (1.3%) for I. verum; methyleugenol (81.2%) and safrole (10.6%) for O. odorifera; and safrole (82.5%) and α-terpinolene (13.4%) for P. hispidinervum. The acute toxicity test (24 hours) performed by contact in sorghum leaves or contaminated filter-paper showed that the higher toxicity occur with essential oils of A. conyzoides, with LC50of 7.01 and 7.01 µg oil/cm2, respectively; followed by O. odorifera, with LC50 of 17.10 and 66.70 µg oil/cm2, respectively; I. verum, with LC50of 66.40 and 65.40 µg oil/cm2, respectively; and low toxicity was observed for the P. hispidinervum essential oil, with LC50 of 64.00 and 143.00 oil/cm2, respectively. With the results obtained in this research, we can state that the use of these essential oils can be a new tool in integrated pest management.

This study demonstrates the possibility of using sanitizing detergents based on natural products for the elimination and/or reduction of Aeromonas hydrophila biofilm formed on stainless steel surfaces. The goal of this work was to determine the reduction effect of sanitizing detergents containing essential oils of Thymus vulgaris (thyme) and Cymbopogon citratus (lemongrass) on biofilm formed by A. hydrophila on AISI 304 stainless steel coupons, using UHT skimmed milk as substratum. There was adhesion and biofilm formation by A. hydrophila at 28 ºC, presenting 7.60 log cfu.cm-2 after the fourth day of cultivation. There was no significant difference between the lemongrass treatment and that of the thyme oil (p < 0.05). However, both treatments significantly reduced the biofilm, differing significantly from the NaOH control (p > 0.05). The treatment with lemongrass solution reduced the biofilm by 4.51 log cfu cm-2 at 25 ºC. The thyme detergent also reduced the number of cfu cm-2 by 3.84 log cycles at 25 ºC. The use of the lemongrass and thyme solutions efficiently reduced the A. hydrophila biofilm.

This research evaluated the antimicrobial effect of the clove (Syzygium aromaticum) and lemongrass (Cymbopogon citratus (DC.) Stapf.) essential oils (EOs) against Listeria monocytogenes ATCC 19117 growth added to bovine ground meat stored under refrigeration (5 ± 2 °C) for three days. The EOs, extracted by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS), were tested in vitro using an agar well diffusion methodology for determination of Minimum Inhibitory Concentration (MIC). The MIC concentrations for both essential oils on culture tested of L. monocytogenes were 1.56%. The EOs concentrations applied in contaminated ground beef were 1.56, 3.125 and 6.25% (w/v) based on MIC levels and possible activity reductions by food constituents. The bacteria populations were significantly reduced (p < 0.05) after one day of storage in ground meat samples treated with clove and lemongrass EOs at concentrations of 1.56%. There were no significant counts of L. monocytogenes in samples at the other concentrations of the two oils applied after the second day of storage. The sensory acceptability evaluation of the bovine ground meat samples treated with EOs showed that the addition at concentrations higher than 1.56% promote undesirable alterations of taste, odor and characteristic color. The application of EOs at low concentrations in food products can be used in combination with other preservation methods, such as refrigeration, to control pathogens and spoilage bacteria during shelf-life; which goes according to current market trends, where consumers are requesting natural products.

A nogueira-macadâmia produz nozes de alto valor no mercado internacional, devido às características nutricionais de suas amêndoas, consideradas uma excelente fonte energética. O objetivo do trabalho foi realizar a caracterização química e o perfil de ácidos graxos em 22 cultivares de nogueira-macadâmia. As cultivares utilizadas foram: 'Edson', 'HAES 788 (Pahala)', 'Beaumont 695', 'Flor Rosa', 'IAC 9-20X', 'HAES 344 (Kau)', 'Cannon', 'IAC 9-20', 'C160', 'HAES 849', 'IAC 4-12B', 'HAES 816', 'Doroti', '791 Fuji', 'IAC 4-20 (Keaumi)', 'HAES 814', 'HAES 722', 'África', 'IAC Campinas-B', 'HAES 246 (Keauhou)', 'HAES 741 (Mauka)' e 'HAES 842'. Foram quantificados a umidade, extrato etéreo, proteína, cinza e fibra bruta, além disso, extração de óleo e o perfil de ácidos graxos. A maior quantidade de proteína foi registrada na cultivar 'IAC 9-20X'. Quanto ao teor de fibra bruta, as maiores porcentagens foram observadas nas cultivares 'HAES 741' e 'HAES 842'. As amêndoas de todas as cultivares de nogueira-macadâmia analisadas possuem ácidos palmítico e oleico.

The walnuts macadamia have a high value on the international market due to their almonds nutritional characteristics considered to be an excellent source of energy. The aim of this work was to evaluate the chemical composition and fatty acid profile of 22 cultivars of almond walnut macadamia. The cultivars tested were: 'Edson', 'HAES 788 (Pahala)', 'Beaumont 695', 'Flor Rosa', 'IAC 9-20X', 'HAES 344 (Kau)', 'Cannon', 'IAC 9-20', 'C160', 'HAES 849', 'IAC 4-12B', 'HAES 816', 'Doroti', '791 Fuji', 'IAC 4-20 (Keaumi)', 'HAES 814', 'HAES 722', 'África', 'IAC Campinas-B', 'HAES 246 (Keauhou)', 'HAES 741 (Mauka)' and 'HAES 842'. Moisture, fat, protein, ash and crude fiber were analyzed and also oil extraction and fatty acid profile. The highest amount of protein was recorded in 'IAC 9-20X'. The highest percentages of crude fiber content were observed in cultivars 'HAES 741' and 'HAES 842'. The kernels of all walnut macadamia cultivars showed palmitic and oleic acids.